Reassortment between Two Serologically Unrelated Bluetongue Virus Strains Is Flexible and Can Involve any Genome Segment
Identifieur interne : 000142 ( France/Analysis ); précédent : 000141; suivant : 000143Reassortment between Two Serologically Unrelated Bluetongue Virus Strains Is Flexible and Can Involve any Genome Segment
Auteurs : Andrew E. Shaw [Royaume-Uni] ; Maxime Ratinier [Royaume-Uni] ; Sandro Filipe Nunes [Royaume-Uni] ; Kyriaki Nomikou [Royaume-Uni] ; Marco Caporale [Royaume-Uni, Italie] ; Matthew Golder [Royaume-Uni] ; Kathryn Allan [Royaume-Uni] ; Claude Hamers [France] ; Pascal Hudelet [France] ; Stéphan Zientara [France] ; Emmanuel Breard [France] ; Peter Mertens [Royaume-Uni] ; Massimo Palmarini [Royaume-Uni]Source :
- Journal of Virology [ 0022-538X ] ; 2013.
Abstract
Coinfection of a cell by two different strains of a segmented virus can give rise to a “reassortant” with phenotypic characteristics that might differ from those of the parental strains. Bluetongue virus (BTV) is a double-stranded RNA (dsRNA) segmented virus and the cause of bluetongue, a major infectious disease of livestock. BTV exists as at least 26 different serotypes (BTV-1 to BTV-26). Prompted by the isolation of a field reassortant between BTV-1 and BTV-8, we systematically characterized the process of BTV reassortment. Using a reverse genetics approach, our study clearly indicates that any BTV-1 or BTV-8 genome segment can be rescued in the heterologous “backbone.” To assess phenotypic variation as a result of reassortment, we examined viral growth kinetics and plaque sizes in
Url:
DOI: 10.1128/JVI.02266-12
PubMed: 23097432
PubMed Central: 3536370
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000659
- to stream Pmc, to step Curation: 000659
- to stream Pmc, to step Checkpoint: 000576
- to stream Ncbi, to step Merge: 000829
- to stream Ncbi, to step Curation: 000829
- to stream Ncbi, to step Checkpoint: 000829
- to stream Main, to step Merge: 000842
- to stream Main, to step Curation: 000841
- to stream Main, to step Exploration: 000841
- to stream France, to step Extraction: 000142
Links to Exploration step
PMC:3536370Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Reassortment between Two Serologically Unrelated Bluetongue Virus Strains Is Flexible and Can Involve any Genome Segment</title>
<author><name sortKey="Shaw, Andrew E" sort="Shaw, Andrew E" uniqKey="Shaw A" first="Andrew E." last="Shaw">Andrew E. Shaw</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow</wicri:regionArea>
<wicri:noRegion>Glasgow</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ratinier, Maxime" sort="Ratinier, Maxime" uniqKey="Ratinier M" first="Maxime" last="Ratinier">Maxime Ratinier</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow</wicri:regionArea>
<wicri:noRegion>Glasgow</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nunes, Sandro Filipe" sort="Nunes, Sandro Filipe" uniqKey="Nunes S" first="Sandro Filipe" last="Nunes">Sandro Filipe Nunes</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow</wicri:regionArea>
<wicri:noRegion>Glasgow</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nomikou, Kyriaki" sort="Nomikou, Kyriaki" uniqKey="Nomikou K" first="Kyriaki" last="Nomikou">Kyriaki Nomikou</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">The Pirbright Institute, Pirbright, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Pirbright Institute, Pirbright</wicri:regionArea>
<wicri:noRegion>Pirbright</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Caporale, Marco" sort="Caporale, Marco" uniqKey="Caporale M" first="Marco" last="Caporale">Marco Caporale</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow</wicri:regionArea>
<wicri:noRegion>Glasgow</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff3">Istituto G. Caporale, Teramo, Italy</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Istituto G. Caporale, Teramo</wicri:regionArea>
<wicri:noRegion>Teramo</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Golder, Matthew" sort="Golder, Matthew" uniqKey="Golder M" first="Matthew" last="Golder">Matthew Golder</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow</wicri:regionArea>
<wicri:noRegion>Glasgow</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Allan, Kathryn" sort="Allan, Kathryn" uniqKey="Allan K" first="Kathryn" last="Allan">Kathryn Allan</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow</wicri:regionArea>
<wicri:noRegion>Glasgow</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hamers, Claude" sort="Hamers, Claude" uniqKey="Hamers C" first="Claude" last="Hamers">Claude Hamers</name>
<affiliation wicri:level="3"><nlm:aff id="aff4">Merial S.A.S., Lyon, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Merial S.A.S., Lyon</wicri:regionArea>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hudelet, Pascal" sort="Hudelet, Pascal" uniqKey="Hudelet P" first="Pascal" last="Hudelet">Pascal Hudelet</name>
<affiliation wicri:level="3"><nlm:aff id="aff4">Merial S.A.S., Lyon, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Merial S.A.S., Lyon</wicri:regionArea>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zientara, Stephan" sort="Zientara, Stephan" uniqKey="Zientara S" first="Stéphan" last="Zientara">Stéphan Zientara</name>
<affiliation wicri:level="1"><nlm:aff id="aff5">French Agency for Food, Environment and Occupational Health and Safety (ANSES), Maisons-Alfort, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>French Agency for Food, Environment and Occupational Health and Safety (ANSES), Maisons-Alfort</wicri:regionArea>
<wicri:noRegion>Maisons-Alfort</wicri:noRegion>
<wicri:noRegion>Maisons-Alfort</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Breard, Emmanuel" sort="Breard, Emmanuel" uniqKey="Breard E" first="Emmanuel" last="Breard">Emmanuel Breard</name>
<affiliation wicri:level="1"><nlm:aff id="aff5">French Agency for Food, Environment and Occupational Health and Safety (ANSES), Maisons-Alfort, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>French Agency for Food, Environment and Occupational Health and Safety (ANSES), Maisons-Alfort</wicri:regionArea>
<wicri:noRegion>Maisons-Alfort</wicri:noRegion>
<wicri:noRegion>Maisons-Alfort</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mertens, Peter" sort="Mertens, Peter" uniqKey="Mertens P" first="Peter" last="Mertens">Peter Mertens</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">The Pirbright Institute, Pirbright, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Pirbright Institute, Pirbright</wicri:regionArea>
<wicri:noRegion>Pirbright</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Palmarini, Massimo" sort="Palmarini, Massimo" uniqKey="Palmarini M" first="Massimo" last="Palmarini">Massimo Palmarini</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow</wicri:regionArea>
<wicri:noRegion>Glasgow</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">23097432</idno>
<idno type="pmc">3536370</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3536370</idno>
<idno type="RBID">PMC:3536370</idno>
<idno type="doi">10.1128/JVI.02266-12</idno>
<date when="2013">2013</date>
<idno type="wicri:Area/Pmc/Corpus">000659</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000659</idno>
<idno type="wicri:Area/Pmc/Curation">000659</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000659</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000576</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000576</idno>
<idno type="wicri:Area/Ncbi/Merge">000829</idno>
<idno type="wicri:Area/Ncbi/Curation">000829</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000829</idno>
<idno type="wicri:doubleKey">0022-538X:2013:Shaw A:reassortment:between:two</idno>
<idno type="wicri:Area/Main/Merge">000842</idno>
<idno type="wicri:Area/Main/Curation">000841</idno>
<idno type="wicri:Area/Main/Exploration">000841</idno>
<idno type="wicri:Area/France/Extraction">000142</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Reassortment between Two Serologically Unrelated Bluetongue Virus Strains Is Flexible and Can Involve any Genome Segment</title>
<author><name sortKey="Shaw, Andrew E" sort="Shaw, Andrew E" uniqKey="Shaw A" first="Andrew E." last="Shaw">Andrew E. Shaw</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow</wicri:regionArea>
<wicri:noRegion>Glasgow</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ratinier, Maxime" sort="Ratinier, Maxime" uniqKey="Ratinier M" first="Maxime" last="Ratinier">Maxime Ratinier</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow</wicri:regionArea>
<wicri:noRegion>Glasgow</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nunes, Sandro Filipe" sort="Nunes, Sandro Filipe" uniqKey="Nunes S" first="Sandro Filipe" last="Nunes">Sandro Filipe Nunes</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow</wicri:regionArea>
<wicri:noRegion>Glasgow</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nomikou, Kyriaki" sort="Nomikou, Kyriaki" uniqKey="Nomikou K" first="Kyriaki" last="Nomikou">Kyriaki Nomikou</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">The Pirbright Institute, Pirbright, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Pirbright Institute, Pirbright</wicri:regionArea>
<wicri:noRegion>Pirbright</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Caporale, Marco" sort="Caporale, Marco" uniqKey="Caporale M" first="Marco" last="Caporale">Marco Caporale</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow</wicri:regionArea>
<wicri:noRegion>Glasgow</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff3">Istituto G. Caporale, Teramo, Italy</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Istituto G. Caporale, Teramo</wicri:regionArea>
<wicri:noRegion>Teramo</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Golder, Matthew" sort="Golder, Matthew" uniqKey="Golder M" first="Matthew" last="Golder">Matthew Golder</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow</wicri:regionArea>
<wicri:noRegion>Glasgow</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Allan, Kathryn" sort="Allan, Kathryn" uniqKey="Allan K" first="Kathryn" last="Allan">Kathryn Allan</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow</wicri:regionArea>
<wicri:noRegion>Glasgow</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hamers, Claude" sort="Hamers, Claude" uniqKey="Hamers C" first="Claude" last="Hamers">Claude Hamers</name>
<affiliation wicri:level="3"><nlm:aff id="aff4">Merial S.A.S., Lyon, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Merial S.A.S., Lyon</wicri:regionArea>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hudelet, Pascal" sort="Hudelet, Pascal" uniqKey="Hudelet P" first="Pascal" last="Hudelet">Pascal Hudelet</name>
<affiliation wicri:level="3"><nlm:aff id="aff4">Merial S.A.S., Lyon, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Merial S.A.S., Lyon</wicri:regionArea>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Lyon</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zientara, Stephan" sort="Zientara, Stephan" uniqKey="Zientara S" first="Stéphan" last="Zientara">Stéphan Zientara</name>
<affiliation wicri:level="1"><nlm:aff id="aff5">French Agency for Food, Environment and Occupational Health and Safety (ANSES), Maisons-Alfort, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>French Agency for Food, Environment and Occupational Health and Safety (ANSES), Maisons-Alfort</wicri:regionArea>
<wicri:noRegion>Maisons-Alfort</wicri:noRegion>
<wicri:noRegion>Maisons-Alfort</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Breard, Emmanuel" sort="Breard, Emmanuel" uniqKey="Breard E" first="Emmanuel" last="Breard">Emmanuel Breard</name>
<affiliation wicri:level="1"><nlm:aff id="aff5">French Agency for Food, Environment and Occupational Health and Safety (ANSES), Maisons-Alfort, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>French Agency for Food, Environment and Occupational Health and Safety (ANSES), Maisons-Alfort</wicri:regionArea>
<wicri:noRegion>Maisons-Alfort</wicri:noRegion>
<wicri:noRegion>Maisons-Alfort</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mertens, Peter" sort="Mertens, Peter" uniqKey="Mertens P" first="Peter" last="Mertens">Peter Mertens</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">The Pirbright Institute, Pirbright, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Pirbright Institute, Pirbright</wicri:regionArea>
<wicri:noRegion>Pirbright</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Palmarini, Massimo" sort="Palmarini, Massimo" uniqKey="Palmarini M" first="Massimo" last="Palmarini">Massimo Palmarini</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC–University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow</wicri:regionArea>
<wicri:noRegion>Glasgow</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Coinfection of a cell by two different strains of a segmented virus can give rise to a “reassortant” with phenotypic characteristics that might differ from those of the parental strains. Bluetongue virus (BTV) is a double-stranded RNA (dsRNA) segmented virus and the cause of bluetongue, a major infectious disease of livestock. BTV exists as at least 26 different serotypes (BTV-1 to BTV-26). Prompted by the isolation of a field reassortant between BTV-1 and BTV-8, we systematically characterized the process of BTV reassortment. Using a reverse genetics approach, our study clearly indicates that any BTV-1 or BTV-8 genome segment can be rescued in the heterologous “backbone.” To assess phenotypic variation as a result of reassortment, we examined viral growth kinetics and plaque sizes in <italic>in vitro</italic>
experiments and virulence in an experimental mouse model of bluetongue disease. The monoreassortants generated had phenotypes that were very similar to those of the parental wild-type strains both <italic>in vitro</italic>
and <italic>in vivo</italic>
. Using a forward genetics approach in cells coinfected with BTV-1 and BTV-8, we have shown that reassortants between BTV-1 and BTV-8 are generated very readily. After only four passages in cell culture, we could not detect wild-type BTV-1 or BTV-8 in any of 140 isolated viral plaques. In addition, most of the isolated reassortants contained heterologous VP2 and VP5 structural proteins, while only 17% had homologous VP2 and VP5 proteins. Our study has shown that reassortment in BTV is very flexible, and there is no fundamental barrier to the reassortment of any genome segment. Given the propensity of BTV to reassort, it is increasingly important to have an alternative classification system for orbiviruses.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
<li>Italie</li>
<li>Royaume-Uni</li>
</country>
<region><li>Auvergne-Rhône-Alpes</li>
<li>Rhône-Alpes</li>
</region>
<settlement><li>Lyon</li>
</settlement>
</list>
<tree><country name="Royaume-Uni"><noRegion><name sortKey="Shaw, Andrew E" sort="Shaw, Andrew E" uniqKey="Shaw A" first="Andrew E." last="Shaw">Andrew E. Shaw</name>
</noRegion>
<name sortKey="Allan, Kathryn" sort="Allan, Kathryn" uniqKey="Allan K" first="Kathryn" last="Allan">Kathryn Allan</name>
<name sortKey="Caporale, Marco" sort="Caporale, Marco" uniqKey="Caporale M" first="Marco" last="Caporale">Marco Caporale</name>
<name sortKey="Golder, Matthew" sort="Golder, Matthew" uniqKey="Golder M" first="Matthew" last="Golder">Matthew Golder</name>
<name sortKey="Mertens, Peter" sort="Mertens, Peter" uniqKey="Mertens P" first="Peter" last="Mertens">Peter Mertens</name>
<name sortKey="Nomikou, Kyriaki" sort="Nomikou, Kyriaki" uniqKey="Nomikou K" first="Kyriaki" last="Nomikou">Kyriaki Nomikou</name>
<name sortKey="Nunes, Sandro Filipe" sort="Nunes, Sandro Filipe" uniqKey="Nunes S" first="Sandro Filipe" last="Nunes">Sandro Filipe Nunes</name>
<name sortKey="Palmarini, Massimo" sort="Palmarini, Massimo" uniqKey="Palmarini M" first="Massimo" last="Palmarini">Massimo Palmarini</name>
<name sortKey="Ratinier, Maxime" sort="Ratinier, Maxime" uniqKey="Ratinier M" first="Maxime" last="Ratinier">Maxime Ratinier</name>
</country>
<country name="Italie"><noRegion><name sortKey="Caporale, Marco" sort="Caporale, Marco" uniqKey="Caporale M" first="Marco" last="Caporale">Marco Caporale</name>
</noRegion>
</country>
<country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Hamers, Claude" sort="Hamers, Claude" uniqKey="Hamers C" first="Claude" last="Hamers">Claude Hamers</name>
</region>
<name sortKey="Breard, Emmanuel" sort="Breard, Emmanuel" uniqKey="Breard E" first="Emmanuel" last="Breard">Emmanuel Breard</name>
<name sortKey="Hudelet, Pascal" sort="Hudelet, Pascal" uniqKey="Hudelet P" first="Pascal" last="Hudelet">Pascal Hudelet</name>
<name sortKey="Zientara, Stephan" sort="Zientara, Stephan" uniqKey="Zientara S" first="Stéphan" last="Zientara">Stéphan Zientara</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000142 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000142 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= H2N2V1 |flux= France |étape= Analysis |type= RBID |clé= PMC:3536370 |texte= Reassortment between Two Serologically Unrelated Bluetongue Virus Strains Is Flexible and Can Involve any Genome Segment }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/France/Analysis/RBID.i -Sk "pubmed:23097432" \ | HfdSelect -Kh $EXPLOR_AREA/Data/France/Analysis/biblio.hfd \ | NlmPubMed2Wicri -a H2N2V1
This area was generated with Dilib version V0.6.33. |